Isolation of Nutritional Mutants of Neurospora crassa by Filtration Enrichment
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چکیده
منابع مشابه
Isolation of circadian clock mutants of Neurospora crassa.
Three mutants of Neurospora crassa have been isolated which have altered period lengths of their circadian rhythm of conidiation. The strains, designated "frequency" (frq), were obtained after mutagenesis of the band (bd) strain with N-methyl-N'-nitro-N-nitrosoguanidine. In continuous darkness at 25 degrees bd has a period length of 21.6 +/- 0.5 hours; under the same conditions the period lengt...
متن کاملIsolation and characterization of low-kynureninase mutants of Neurospora crassa.
Two kynureninase activities are known in Neurospora crassa, one of which (kynureninase I) is inducible, the other (kynureninase II) being constitutive. A method is described for the isolation of low-kynureninase mutants of N. crassa. When grown on an inducer, the mutants show significantly less kynureninase I activity compared with wild type, whereas constitutive kynureninase II activity is una...
متن کاملIsolation and characterization of light-insensitive mutants of Neurospora crassa.
As part of a genetic analysis of blue light photoreception in Neurospora, three mutants were isolated that do not exhibit photosuppression of circadian conidiation, i.e., they show periodic conidiation in constant light. The mutations have been given the designations lis-1, lis-2 and lis-3 ("light insensitive"). The three mutations segregate as single nuclear genes, are nonallelic and are reces...
متن کاملIsolation, mapping, and characterization of trehalaseless mutants of Neurospora crassa.
Mutant strains of Neurospora crassa that lack trehalase and are unable to grow on trehalose were isolated, and the gene (tre) was positioned on the right arm of linkage group I. Maltase and beta-galactosidase activities are almost identical in tre(-) strains, whereas that of invertase was reduced by more than half and those of acid phosphatase and amylase were somewhat increased. Heterocaryons ...
متن کاملIsolation and properties of selenomethionine-resistant mutants of Neurospora crassa.
Mutants resistant to selenomethionine were isolated, and their properties studied. Mapping studies indicate that the mutation sites are located near the eth-1(r) locus in linkage group I, about ten map units away from the mating type locus. The sites of new mutation are either allelic to or very close to eth-1(r). They are resistant not only to selenomethionine but also to ethionine, while the ...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1954
ISSN: 0022-1287
DOI: 10.1099/00221287-11-1-34